| 货号 | 5134S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | RCC1 (D15H6) Rabbit mAb detects endogenous levels of total RCC1 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle and nuclear transport (1,2). Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. RCC1 (regulator of chromatin condensation 1) is the only known RanGEF (3). RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally (4,5). Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation (5,6) and methylation (7). RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis (8). In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclinB/cdc2 (9-11). This phosphorylation may play a role in RCC1 interaction with chromatin and its RanGEF activity (6). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts from various cell types using RCC1 (D15H6) Rabbit mAb. 使用RCC1 (D15H6) Rabbit mAb，免疫印迹（Western Blot）分析不同细胞中RCC1蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa cells using RCC1 (D15H6) Rabbit mAb (green). Actin filaments were labeled using DY-554 phalloidin (red). 使用RCC1 (D15H6) Rabbit mAb (绿色)标记，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。 |
