Rig-I (D33H10) Rabbit mAb【科瑞奥博】

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Rig-I (D33H10) Rabbit mAb

货号： 4200S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	4200S
反应种属	Human/Mouse
来源宿主	Rabbit
应用	W/IP
目标/特异性	Rig-I (D33H10) Rabbit mAb detects endogenous levels of total Rig-I protein.
使用方法	WB(1:1000) IP (1:50)

性能
供应商	CST
背景	Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes (1-3). TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection (4,5). Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling (4-7). Rig-I binds both dsRNA and viral ssRNA that contains a 5-triphosphate end not seen in host RNA (8,9). Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses (10,11). The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-κB, TBK1/IKKε, and IRF-3/IRF-7 (12-15).
存放说明	-20C
计算分子量	102

参考图片

Western blot analysis of differentiated THP-1 and RAW 264.7 cells, untreated () or LPS-treated (1 mu-g/ml, overnight), using Rig-I (D33H10) Rabbit mAb.Western免疫印迹。用Rig-I (D33H10) Rabbit mAb研究未经处理的和经LPS (1mu-g/ml, 过夜)处理分化了的THP-1 和 RAW 264.7细胞的细胞提取液。

Western blot analysis of COS-7 cells, untransfected () or transfected (+) with human Rig-I, using Rig-I (D33H10) Rabbit mAb.Western免疫印迹。用Rig-I (D33H10) Rabbit mAb研究未经处理的和经Rig-I处理的COS-7细胞的细胞提取液。