| 货号 | 12187S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | RNF40 (D2R2O) Rabbit mAb recognizes endogenous levels of total RNF40 protein. This antibody does not cross-react with RNF20/BRE1A. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | In mammalian cells, the significance of histone H2B ubiquitination in chromatin epigenetics came from the identification of the budding yeast protein Bre1 (1,2). Together with the ubiquitin-conjugating enzyme Rad6, Bre1 serves as the E3 ligase in the monoubiquitination of the yeast histone H2B within transcribed regions of chromatin (1-3). Subsequently, the mammalian orthologs of yeast Bre1, RNF20 and RNF40, were identified (4,5). These two proteins form a tight heterodimer that acts as the major E3 ligase responsible for histone H2B monoubiquitination at Lys120 in mammalian cells, a modification linked to RNA Pol II-dependent transcription elongation in undamaged cells. Researchers have shown that DNA double-strand breaks (DSBs) are also capable of inducing monoubiquitination of H2B. This process depends upon the recruitment to DSB sites, as well as ATM-dependent phosphorylation of the RNF20-RNF40 heterodimer, thus highlighting a role for this E3 ligase in DSB repair pathways (6). Indeed, investigators have shown that loss of RNF20-RNF40 function promotes replication stress and chromosomal instability, which may constitute an early step in malignant transformation that precedes cell invasion (7). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Western blot analysis of extracts from various cell lines using RNF40 (D2R2O) Rabbit mAb. Western blot方法检测不同细胞系的提取物，所用抗体为RNF40 (D2R2O) mAb兔单抗。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human RNF20 (hRNF20-Myc/DDK; +) and Myc/DDK-tagged full-length human RNF40 (hRNF40-Myc/DDK; +), using RNF40 (D2R2O) Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower). Western blot方法检测293T细胞提取物，分别用模拟物（-）或构建的带有Myc/DDK标签的全长人M RNF20 (hRNF20-Myc/DDK; +)以及带 Myc/DDK标签的全长人RNF40 (hRNF40-Myc/DDK; +)，使用的抗体为 RNF40 (D2R2O) Rabbit mAb 兔单抗(上图) 以及DYKDDDDK Tag Antibody #2368 (下图)。 | |
Immunoprecipitation of RNF40 from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or RNF40 (D2R2O) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using RNF40 (D2R2O) Rabbit mAb. 从293T细胞提取物中免疫沉淀RNF40，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane2)或RNF40 (D2R2O) Rabbit mAb (lane 3)兔单抗。Lane 1为10% input。 使用RNF40 (D2R2O) Rabbit mAb兔单抗进行Western blot检测。 |
