| 货号 | 9029S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | ROCK2 (D1B1) Rabbit mAb recognizes endogenous levels of total ROCK2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB) activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase (3). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction (4-6). |
| 存放说明 | -20C |
| 计算分子量 | 160 |
Western blot analysis of extracts from various cell lines using ROCK2 (D1B1) Rabbit mAb.使用ROCK2 (D1B1) Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of ROCK2 from HCT-15 cell extracts using ROCK2 (D1B1) Rabbit mAb (lane 2). Western blot was performed using the same antibody. Lane 1 is 10% input.使用ROCK2 (D1B1) Rabbit mAb（lane2）对HCT-15细胞提取物中的ROCK2进行免疫沉淀实验。同样的抗体也被用于western blot。Lane1 是10%的上样量。 |
