| 货号 | 13089S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | RUNX3/AML2 (D9K6L) Mouse mAb recognizes endogenous levels of total RUNX3 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Runt-related transcription factor 3 (RUNX3, AML2), a member of the Runt family of transcription factors, plays an important role in the suppression of gastric epithelium cell proliferation (1), dorsal root ganglia neurogenesis (2), and T cell differentiation (3,4). RUNX3 is also involved in caspase-3-dependent apoptosis (5). Protein complexes containing RUNX3 and various transcription factors, such as Smads or β-catenin/TCF4, have tumor suppressor activity and regulate downstream target gene transcription (6,7). While typically localized to the nucleus, RUNX3 can be tyrosine phosphorylated and located in the cytoplasm of many cancer cells. This mislocalization of RUNX3 abolishes its tumor suppressor function and contributes to tumorigenesis (8). Research studies indicate that gene silencing or protein mislocalization inactivates RUNX3 in more than 80% of gastric cancers and other cancer types (1,9,10). |
| 存放说明 | -20C |
| 计算分子量 | 43-48 |
Confocal immunofluorescent analysis of SW620 (left) and MCF7 (right) cells using RUNX3/AML2 (D9K6L) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用RUNX3/AML2 (D9K6L) Mouse mAb (绿色)对SW620 (左) 和MCF7 (右)细胞进行共聚焦免疫荧光分析。肌动蛋白丝使用DY-554 phalloidin(红色)进行标记。蓝色伪彩= DRAQ5® #4084 (DNA荧光染色)。 | |
Western blot analysis of extracts from SW620, HT-1080, and MCF7 cells using RUNX3/AML2 (D9K6L) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). 使用RUNX3/AML2 (D9K6L) Mouse mAb (上)和 β-Actin (D6A8) Rabbit mAb #8457 (下)对SW620, HT-1080和MCF7细胞提取物进行western blot分析。 |
