| 货号 | 3336S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | S5a/PSMD4 (D17E4) Rabbit mAb recognizes endogenous levels of total S5a/PSMD4 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | S5a (PSMD4) is a subunit of the 19S regulatory proteasome complex functioning in ubiquitinated-protein targeting and degradation (1). S5a contains two polyubiquitin binding motifs (UIM) that bind multiubiquitin chains by hydrophobic interaction (2,3). In addition to ubiquitin, the UIM of S5a shows high affinity to a ubiquitin-like domain present in many proteins. S5a binds to these types of proteins directly and mediates their targeting to the proteasome for degradation (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Western blot analysis of extracts from various cell lines using S5a/PSMD4 (D17E4) Rabbit mAb. Western blot检测多种细胞提取物。 | |
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human S5a/PSMD4 (hS5a/PSMD4-Myc/DDK, +), using S5a/PSMD4 (D17E4) Rabbit mAb. Western blot检测mock transfected (-)或转染表达Myc/DDK-tagged cDNA编码人全长S5a/PSMD4 (hS5a/PSMD4-Myc/DDK, +)的293T细胞，采用S5a/PSMD4 (D17E4)Rabbit mAb。 |
