| 货号 | 12441S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | S5a/PSMD4 (D20B2) Rabbit mAb recognizes endogenous levels of total S5a/PSMD4 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | S5a (PSMD4) is a subunit of the 19S regulatory proteasome complex functioning in ubiquitinated-protein targeting and degradation (1). S5a contains two polyubiquitin binding motifs (UIM) that bind multiubiquitin chains by hydrophobic interaction (2,3). In addition to ubiquitin, the UIM of S5a shows high affinity to a ubiquitin-like domain present in many proteins. S5a binds to these types of proteins directly and mediates their targeting to the proteasome for degradation (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Western blot analysis of extracts from various cell lines using S5a/PSMD4 (D20B2) Rabbit mAb.Western blot检测多种细胞提取物。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human S5a/PSMD4 (hS5a/PSMD4-Myc/DDK; +), using S5a/PSMD4 (D20B2) Rabbit mAb. Western blot检测mock transfected (-)或转染表达Myc/DDK-tagged人全长S5a/PSMD4 (hS5a/PSMD4-Myc/DDK; +)293T细胞，采用S5a/PSMD4 (D20B2) Rabbit mAb。 | |
Immunoprecipitation of S5a/PSMD4 from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or S5a/PSMD4 (D20B2) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using S5a/PSMD4 (D20B2) Rabbit mAb.Immunoprecipitation 检测293T细胞提取物种的S5a/PSMD4，采用Rabbit (DA1E) mAb IgG XP® 同型对照#3900 (lane 2) 或S5a/PSMD4 (D20B2) Rabbit mAb (lane 3)。Lane 1中样品浓度为10%。Western blot检测采用S5a/PSMD4 (D20B2) Rabbit mAb。 |
