S6 Ribosomal Protein (54D2) Mouse mAb【科瑞奥博】

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S6 Ribosomal Protein (54D2) Mouse mAb

货号： 2317S 基本售价： 3360.0 元规格： 100ul

产品信息

概述
货号	2317S
反应种属	Human/Mouse/Rat/Monkey/D.melanogaster
来源宿主	Mouse
应用	W/IP/IHC-P/IF-IC/F
目标/特异性	S6 Ribosomal Protein (54D2) Mouse mAb detects endogenous levels of total S6 ribosomal protein independent of phosphorylation.
使用方法	WB(1:1000) IP (1:100) IHC-P (1:100) F (1:25) IF-IC (1:25)

性能
供应商	CST
背景	One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
存放说明	-20C
计算分子量	32

参考图片

Flow cytometric analysis of NIH/3T3 cells, using S6 Ribosomal Protein (54D2) Mouse mAb (blue) compared to a nonspecifc negative control antibody (red).

与非特异阴性对照抗体 (红色)比较，使用S6 Ribosomal Protein (54D2) Mouse mAb鼠单抗(蓝色)标记，流式细胞仪分析NIH/3T3细胞。

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using S6 Ribosomal Protein (54D2) Mouse mAb.

使用S6 Ribosomal Protein (54D2) Mouse mAb鼠单抗，免疫组化分析人类乳腺癌石蜡切片，结果显示S6 Ribosomal Protein(54D2)蛋白在细胞质定位。

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using S6 Ribosomal Protein (54D2) Mouse mAb.

使用S6 Ribosomal Protein (54D2) Mouse mAb鼠单抗，免疫组化分析人类结肠癌石蜡切片。

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using S6 Ribosomal Protein (54D2) Mouse mAb.

使用S6 Ribosomal Protein (54D2) Mouse mAb鼠单抗，免疫组化分析人类肺癌石蜡切片。

Immunohistochemical analysis of paraffin-embedded LNCaP cells, using S6 Ribosomal Protein (54D2) Mouse mAb.

使用S6 Ribosomal Protein (54D2) Mouse mAb，免疫组化分析LNCaP细胞石蜡切片。

Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using S6 Ribosomal Protein (54D2) Mouse mAb.

使用S6 Ribosomal Protein (54D2) Mouse mAb，免疫印迹（Western Blot）分析在HeLa、NIH/3T3、PC12和COS细胞系中S6 Ribosomal Protein (54D2)蛋白水平。

Confocal immunofluorescent images of HeLa cells labeled with S6 Ribosomal Protein (54D2) Mouse mAb (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

与isotype control (右图)比较，使用S6 Ribosomal Protein (54D2) Mouse mAb (红色，左图)，共聚焦免疫荧光观察S6 Ribosomal Protein (54D2)蛋白在HeLa细胞中定位。fluorescein phalloidin标记微丝蛋白。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。