Sara (D5X4F) Rabbit mAb【科瑞奥博】

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Sara (D5X4F) Rabbit mAb

货号： 13285S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	13285S
反应种属	Human
来源宿主	Rabbit
应用	W/IP
目标/特异性	Sara (D5X4F) Rabbit mAb recognizes endogenous levels of total Sara protein. This antibody also recognizes a non-specific band of unknown origin at 50 kDa.
使用方法	WB(1:1000) IP (1:100)

性能
供应商	CST
背景	The Smad anchor for receptor activation (SARA, ZFYVE9) protein is an FYVE domain-containing protein originally identified as a regulator of TGF-β signaling (1). FYVE domains are zinc finger-like domains that bind to phosphatidylinositol 3-phosphate and are responsible for endosomal trafficking (2). While the role of Sara in TGF-β signaling has been questioned (3,4), early research studies demonstrate that Sara enhances TGF-β signaling by binding and recruiting non-activated Smad2 and Smad3 to the TGF-β receptor complex (1). Upon Smad2 activation, Sara dissociates from the complex while phosphorylated Smad2/3 translocates to the nucleus to bind to the common Smad, Smad4. Sara can also function as an anchor for the protein phosphatase 1 (PP1c) catalytic subunit, which is involved in the Smad7-mediated dephosphorylation of TGF-β type I receptor (5,6). Additional research studies show that expression of Sara plays a critical role in maintenance of the epithelial cell phenotype and that expression is regulated during the epithelial-to-mesenchymal transition (EMT) and fibrosis (7,8).
存放说明	-20C
计算分子量	190, 210

参考图片

Western blot analysis of extracts from various cell lines using Sara (D5X4F) Rabbit mAb.

使用Sara (D5X4F) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human Sara protein (hSara; +), using Sara (D5X4F) Rabbit mAb.

对293T细胞空载体转染(-)，或转染DDK/Myc标签的全长人Sara蛋白质粒(hSara; +)后获取细胞提取物，使用Sara (D5X4F) Rabbit mAb兔单抗进行western blot分析。

Immunoprecipitation of Sara from SH-SY5Y cells using Rabbit (DA1E) mAb IgG XP^®; Isotype Control #3900 (lane 2) or Sara (D5X4F) Rabbit mAb (lane 3). Lane 1 represents 10% input. Western blot was performed using Sara (D5X4F) Rabbit mAb.

使用Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (第 2泳道) 或Sara (D5X4F) Rabbit mAb (第 3泳道)对SH-SY5Y细胞提取物中的Sara蛋白进行免疫沉淀实验。泳道1为10%上样量。使用Sara (D5X4F) Rabbit mAb兔单抗进行western blot分析。

Western blot analysis of extracts from SH-SY5Y cells, transfected with 100 nM SignalSilence^® Control siRNA (Unconjugated) #6568 (-), SignalSilence^® Sara siRNA I (+) #13542, or SignalSilenc^® Sara siRNA II #13553 (+), using Sara (D5X4F) Rabbit mAb (upper) or α-Actinin (D6F6) XP^® Rabbit mAb #6487 (lower). The Sara (D5X4F) Rabbit mAb confirms silencing of Sara expression, while the α-Actinin (D6F6) XP^® Rabbit mAb is used as a loading control.

使用Sara (D5X4F) Rabbit mAb兔单抗（上）或α-Actinin (D6F6) XP^® Rabbit mAb兔单抗 #6487（下）对转染了100 nM SignalSilence^® Control siRNA (无偶联) #6568 (-), SignalSilence^® Sara siRNA I (+), 或SignalSilence^® Sara siRNA II #13553 (+)的SH-SY5Y细胞提取物进行western blot分析。Sara (D5X4F) Rabbit mAb兔单抗用于确认Sara被敲除，α-Actinin (D6F6) XP^® Rabbit mAb 用于确认上样量一致。