| 货号 | 12892S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | <p>The serum response factor (SRF) is a ubiquitous protein that modulates transcription of genes containing serum response elements (SRE) at their promoters. SRF regulates cellular processes such as cell proliferation and cytoskeletal signaling in conjunction with a variety of cofactors (1-3).</p><p>Suppressor of cancer cell invasion (SCAI) is a highly conserved transcriptional cofactor that inhibits the activity of myocardin-related transcription factor (MRTF) family members MAL (MRTF-A), myocardin and OTT-MAL (4,5). SCAI controls the expression of integrin β1 and regulates cell migration and invasion <i>in vitro</i> (4). SCAI has also been shown to play a role in transcriptional regulation in neurons by regulating dendritic morphology through inhibition of the megakaryoblastic leukemia (MKL) family of transcription cofactors (6). Research studies have implicated SCAI in the regulation of the epithelial-to-mesenchymal transition (EMT) as well as in renal fibrosis (7).</p>血清应激因子（SRF）是一个广泛表达的蛋白，可以调节启动子上带有血清应激元件（SRE）的基因转录。SRF和细胞增殖和细胞骨架信号的多种辅助因子相互作用协同调节细胞生命活动（1-3）。 癌细胞侵袭抑制子（SCAI）是一个保守的转录辅助因子，能够抑制myocardin相关转录因子（MRTF）家族成员MAL (MRTF-A), myocardin和OTT-MAL 的活性(4,5)。体外实验中SCAI控制整合素β1的表达以及调控细胞迁移和侵袭（4）。SCAI也被证明在神经元忠发挥了转录调控的作用，这是通过抑制巨核细胞白血病(MKL)家族辅助转录因子来调节树突形态而发挥作用的（6）。研究提示SCAI也在肾脏纤维化的上皮细胞间质化转化(EMT)过程中发挥作用（7）。 |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Western blot analysis of extracts from various cell lines using SCAI (D9A7Y) Rabbit mAb.使用SCAI (D9A7Y) Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of SCAI from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SCAI (D9A7Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using SCAI (D9A7Y) Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 SCAI (D9A7Y) Rabbit mAb (lane 3)对MCF7细胞的SCAI进行免疫沉淀实验。Lane1是10%上样量。使用SCAI (D9A7Y) Rabbit mAb进行western blot分析。 |
