| 货号 | 2794S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | SCD1 (C12H5) Rabbit mAb detects endogenous levels of total SCD1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Stearoyl-CoA desaturase 1 (SCD1) is a key lipogenic enzyme found in the endoplasmic reticulum that catalyzes the conversion of palmitoyl–CoA and stearoyl–CoA to palmitoleoyl–CoA (16:1) and oleoyl–CoA (18:1) (1-3). Palmitoleate and oleate are the major components of triglycerides, membrane phospholipids and cholesterol esters (1). SCD1-knockout mice show improved insulin sensitivity and reduced body fat (1). Disruption of SCD1 in mouse brown adipose tissue strengthens insulin signaling and results in increased translocation of Glut4 to plasma membrane and enhanced uptake of glucose (4). Furthermore, SCD1 is essential for the onset of diet-induced body weight gain (1) and insulin resistance in the liver (5). |
| 存放说明 | -20C |
| 计算分子量 | 37 |
Western blot analysis of 3T3-L1 cell lysates using SCD1 (C12H5) Rabbit mAb. 对3T3-L1细胞裂解液使用SCD1 (C12H5) Rabbit mAb兔单抗进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded mouse brown fat using SCD1 (C12H5) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 对石蜡包埋的小鼠棕色脂肪使用SCD1 (C12H5) Rabbit mAb兔单抗进行免疫组织化学分析，其中左图对照肽，右图抗原特异肽。 | |
Immunohistochemical analysis of paraffin-embedded mouse lung using SCD1 (C12H5) Rabbit mAb (left) or without primary antibody (right). 对石蜡包埋的小鼠肺使用SCD1 (C12H5) Rabbit mAb兔单抗（左）或没有一抗（右）进行免疫组织化学分析。 | |
Immunohistochemical analysis of paraffin-embedded 3T3-L1 cells undifferentiated (left) or differentiated (right) using SCD1 (C12H5) Rabbit mAb. 对石蜡包埋的3T3-L1细胞，未分化（左）或分化的（右），使用SCD1 (C12H5) Rabbit mAb兔单抗进行免疫组化分析。 | |
Confocal immunofluorescent analysis of NIH/3T3-L1 cells, using SCD1 (C12H5) Antibody (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对NIH/3T3-L1细胞使用SCD1 (C12H5) Antibody（红色）进行共聚焦免疫荧光分析，显示分化细胞的胞质定位。脂质小滴被BODIPY 493/503 (绿色)标记。蓝色伪彩=DRAQ5?#4084(荧光DNA染料)。 |
