| 货号 | 12042S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Sec24B (D7D6S) Rabbit mAb recognizes endogenous levels of total Sec24B protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Coat Protein Complex II (COPII) is composed of five cytosolic proteins: Sec23/24 complex, Sec13/31 complex, and Sar1. COPII coat is located at the ER/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23/24 complex, thereby forming a prebudding complex that directly binds target molecules (1-3). The prebudding complex further recruits Sec13/31 to form mature COPII coat (4,5). The Sec24 subunit of COPII coat is thought to play a critical role in cargo selection (2,6). It binds directly to cargo proteins at the ER and brings them to COPII vesicles through interaction with Sec23. There are four Sec24 isoforms in human cells: Sec24A, Sec24B, Sec24C, and Sec24D (7). In mice, mutations in Sec24B have been linked to developmental defects (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Western blot analysis of extracts from various cell lines using Sec24B (D7D6S) Rabbit mAb.使用Sec24B (D7D6S) Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of Sec24B from HeLa cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Sec24B (D7D6S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Sec24B (D7D6S) Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或Sec24B (D7D6S) Rabbit mAb(lane 3)对HeLa细胞提取物中的Sec24B进行免疫沉淀实验。Lane1是10%上样量。使用Sec24B (D7D6S) Rabbit mAb进行western blot分析。 | |
Confocal immunofluorescent analysis of HeLa cells, transfected with Signal Silence® Control siRNA (Unconjugated) #6568 (left), SignalSilence® Sec24B siRNA I #12229 (center), or SignalSilence® Sec24B siRNA II #12333 (right), using Sec24B (D7D6S) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).对转染了ignal Silence® Control siRNA (Unconjugated) #6568（左），SignalSilence® Sec24B siRNA I #12229（中），或SignalSilence® Sec24B siRNA II #12333（右）的HeLa细胞，使用Sec24B (D7D6S) Rabbit mAb（绿色）进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye).。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Sec24B siRNA I #12229 (+), or SignalSilence® Sec24B siRNA II #12333 (+), using Sec24B Antibody #7427 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The Sec24B Antibody confirms silencing of Sec24B expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.对转染了ignal Silence® Control siRNA (Unconjugated) #6568（-），SignalSilence® Sec24B siRNA I #12229（+），或SignalSilence® Sec24B siRNA II #12333（+）的HeLa细胞提取物，使用Sec24B 抗体#7427(上)或β-Actin (D6A8) Rabbit mAb #8457（下）进行Western blot分析。Sec24B 抗体用于确认Sec24B的敲除，β-Actin (D6A8) Rabbit mAb用作上样对照。 |
