货号 | 13445S |
反应种属 | Human |
来源宿主 | Rabbit |
应用 | W/IP |
使用方法 | WB(1:1000) IP (1:200) |
供应商 | CST |
存放说明 | -20C |
计算分子量 | 25 |
Immunoprecipitation of securin from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Securin (D2B6O) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Securin (D2B6O) Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)兔单抗或 Securin (D2B6O) Rabbit mAb (lane 3)对Jurkat细胞提取物中的securin蛋白进行免疫沉淀实验。Lane1是10%上样量。使用Securin (D2B6O) Rabbit mAb进行western blot实验。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human PTTG1 (securin) protein (hPTTG1-Myc/DDK; +) or Myc/DDK-tagged full-length human PTTG2 (securin 2) protein (hPTTG2-Myc/DDK; +), using Securin (D2B6O) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).模拟转染(-)或转染了表达Myc/DDK标记的full-length human PTTG2 (securin 2) protein (hPTTG2-Myc/DDK; +)或Myc/DDK标记 full-length human PTTG2 (securin 2) protein (hPTTG2-Myc/DDK; +),质粒的293T细胞提取物,使用Securin (D2B6O) Rabbit mAb (上) 或 Myc-Tag (71D10) Rabbit mAb #2278 (下)进行western blot实验。 | |
Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with thymidine (2 mM, 16 hr) followed by Nocodazole #2190 (10 nM, 24 hr; +), using Securin (D2B6O) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).未处理(-)或用thymidine (2 mM, 16 hr)后再用Nocodazole #2190 (10 nM, 24 hr; +)处理的HT-29细胞提取物,使用Securin (D2B6O) Rabbit mAb (上)或 β-Actin (D6A8) Rabbit mAb #8457 (下)。 |