Sestrin-2 (D1B6) Rabbit mAb【科瑞奥博】

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Sestrin-2 (D1B6) Rabbit mAb

货号： 8487S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	8487S
反应种属	Human/Monkey
来源宿主	Rabbit
应用	W/IP
使用方法	WB(1:1000) IP (1:50)

性能
供应商	CST
背景	The Sestrin family of proteins, which comprises sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, plays an important role in regulating the cellular response to oxidative stress. Sestrin-1 and -2, both transcriptional targets of p53, have been shown to activate AMPK activity toward TSC2, ultimately resulting in the inhibition of mTOR (1). Thus, sestrin-1 and -2 link p53 signaling to the regulation of cellular growth (2). Sestrins have also been shown to function as modulators of cellular hydrogen peroxide concentration. Hydrogen peroxide is an important signaling molecule that is highly genotoxic and must therefore be tightly regulated (3). 2-Cys peroxiredoxins are metabolizing enzymes that maintain low cellular concentrations of hydrogen peroxide, but are hyperoxidized to an inactive state by a rapid increase in concentration (4) before quickly being reduced back to their active state by sestrins (5). This allows a hydrogen peroxide signal to temporarily circumvent the protective activity of 2-Cys peroxiredoxins, while minimizing oxidative damage.Sestrin蛋白家族，包括sestrin-1（PA26）、sestrin-2（Hi95）和sestrin-3，在调节细胞的氧化应激反应中起着重要的作用。 sestrin-1和-2，两个都是p53基因的转录靶点，被显示能够激活针对TSC2的AMPK活性，最终导致mTOR抑制（1）。因此，sestrin-1和-2连接p53信号至细胞生长调节（2）。也已证明sestrins能够充当细胞的过氧化氢浓度的调节器。过氧化是一个重要的信号传导分子，具有高度遗传毒性，因此，必须严格进行调控（3）。2-半胱氨酸过氧化物酶是能够保持较低的细胞过氧化氢浓度的代谢酶，但是在迅速地减少并通过sestrins返回到它们的激活状态之前(5)，该酶被迅速增加的过氧化氢浓度氧化到非活化状态(4)。这允许过氧化氢信号暂时规避2-半胱氨酸过氧化物酶的保护性活性，同时最大限度地减少氧化损伤。
存放说明	-20C
计算分子量	56

参考图片

Western blot analysis of extracts from K-562 and 293T cells using Sestrin-2 (D1B6) Rabbit mAb.Western blot 方法检测K-562和293T细胞提取物，使用的抗体为 Sestrin-2 (D1B6) Rabbit mAb。

Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with a construct overexpressing full-length human sestrin-2 (hSestrin-2; +), using Sestrin-2 (D1B6) Rabbit mAb.Western blot 方法检测COS-7细胞提取物，分为空对照转染组（-）和过表达全长人sestrin-2的质粒转染组(hSestrin-2; +), 使用的抗体为Sestrin-2 (D1B6) Rabbit mAb。