| 货号 | 12103S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | SGK1 (D27C11) Rabbit mAb recognizes endogenous levels of total SGK1 protein. This antibody does not cross-react with SGK2 or SGK3. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7).In addition to its membrane channel and carrier functions, SGK1 also regulates a broad range of targets, such as GSK-3, NEDD4, iNOS, AMPAR, PSD95, Tau, NF-κB, CREB, and FKHR-L1 (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 45-60 |
Western blot analysis of extracts from SK-MEL-28, SK-MEL-5, and HSG cells using SGK1 (D27C11) Rabbit mAb. 使用SGK1 (D27C11)Rabbit mAb兔单抗对SK-MEL-28, SK-MEL-5和HSG细胞提取物进行western blot分析。 | |
Immunoprecipitation of SGK1 from SK-MEL-28 cell extracts, using Normal Rabbit IgG #2729 (lane 2) or SGK1 (D27C11) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SGK1 (D27C11) Rabbit mAb. 使用正常兔IgG #2729 (lane 2) 或 SGK1 (D27C11) Rabbit mAb 兔单抗(lane 3)对SK-MEL-28细胞提取物中的SGK1进行免疫沉淀。Lane1是10%上样量。使用SGK1 (D27C11)Rabbit mAb兔单抗进行western blot分析。 |
