| 货号 | 6919S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | SIK2 (D28G3) Rabbit mAb recognizes endogenous levels of total SIK2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Salt-inducible kinase 1 (SIK1) was originally identified as a serine/threonine kinase from adrenocortical tissues of rats on a high salt diet (1). SIK1 is a SNF1/AMPK family kinase capable of autophosphorylation (1). SIK2 is an isoform of SIK1 and is specifically expressed in adipose tissues where it is induced during adipocyte differentiation (2). Studies suggest that SIK2 can phosphorylate human insulin receptor substrate (IRS-1) at Ser794. Along with evidence that SIK2 expression and activity are increased in white adipocytes of diabetic mice, this finding suggests a possible role for SIK2 in regulating insulin signaling in adipocytes and in the development of insulin resistance (2,3). Insulin triggers Akt2-mediated phosphorylation of SIK2 at Ser358 and the resultant kinase activation during post-fasting feeding (4). The activated SIK2 then induces the phosphorylation of Torc2 at Ser171 resulting in translocation of this transcriptional coactivator from the nucleus to cytoplasm where it is degraded through the ubiquitin pathway, leading to inhibition of gluconeogenic gene expression (4). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Western blot analysis of extracts from 3T3-L1 cells and human adipocytes using SIK2 (D28G3) Rabbit mAb. Wetern blot检测3T3-L1 细胞和人脂肪细胞提取物，所用抗体为SIK2 (D28G3) Rabbit mAb兔单抗。 |
