SirT2 (D4O5O) Rabbit mAb
货号：
12650S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 12650S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SirT2 (D4O5O) Rabbit mAb recognizes endogenous levels of total SirT2 protein. This antibody does not cross-react with other sirtuin proteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT2, a mammalian homolog of Sir2, deacetylates α-tubulin at Lys40 and histone H4 at Lys16 and has been implicated in cytoskeletal regulation and progression through mitosis (2,3). SirT2 protein is mainly cytoplasmic and is associated with microtubules and HDAC6, another tubulin deacetylase (2). Deacetylation of α-tubulin decreases its stability and may be required for proper regulation of cell shape, intracellular transport, cell motility, and cell division (2,4). The abundance and phosphorylation state of SirT2 increase at the G2/M transition of the cell cycle, and SirT2 relocalizes to chromatin during mitosis when histone H4 Lys16 acetylation levels decrease (3,5). Overexpression of SirT2 prolongs mitosis, while overexpression of the CDC14B phosphatase results in both decreased phosphorylation and abundance of SirT2, allowing for proper mitotic exit (5). Thus, the deacetylation of both histone H4 and α-tubulin by SirT2 may be critical for proper chromatin and cytoskeletal dynamics required for completion of mitosis. |
| 存放说明 | -20C |
| 计算分子量 | 39, 43 |
参考图片Western blot analysis of extracts from various cell lines using SirT2 (D4O5O) Rabbit mAb.Western blot方法检测多种细胞系的提取物，使用的抗体为SirT2 (D4O5O) Rabbit mAb。 |
Western blot analysis of extracts from SirT2 wild-type (WT) and knockout (KO) mouse brain using SirT2 (D4O5O) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). SirT2 WT and KO mouse brain extracts were kindly provided by Dr. Gizem Donmez, Tufts University School of Medicine.Western blot方法检测SirT2 wild-type (WT) 和 knockout (KO) 小鼠脑组织的提取物，使用的抗体为SirT2 (D4O5O) Rabbit mAb (上图)或GAPDH (D16H11) XP® Rabbit mAb #5174（下图）。SirT2 WT 和 KO 小鼠脑组织提取物由Dr. Gizem Donmez, Tufts University School of Medicine惠赠。 |
