| 货号 | 5490S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | SirT3 (D22A3) Rabbit mAb detects endogenous levels of total SirT3 protein. This antibody recognizes both the uncleaved 45 kDa precursor protein and the cleaved 28 kDa processed protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging (1). SirT3, a mammalian homolog of Sir2, exists in human cells in two forms. The full-length 44 kDa protein localizes to the nucleus, while a processed 28 kDa protein lacking 142 amino terminal residues localizes exclusively to the mitochondria (2-4). The single murine form of SirT3 is equivalent to the processed human SirT3 protein (2). Full-length SirT3 protein is processed in the mitochondrial matrix by the mitochondrial matrix processing peptidase (MMP) (3). Both full-length and processed forms of SirT3 are enzymatically active and de-acetylate histone H3 at Lys9 and histone H4 at Lys16 in vitro (2). SirT3 also de-acetylates Lys642 of acetyl-CoA synthetase 2 (AceCS2) and activates AceCS2 activity in the mitochondria (5). Restricted caloric intake, which is linked to increased lifespan in multiple organisms, increases SirT3 expression in white and brown adipocytes of obese mice, suggesting a role for SirT3 in aging (6). Two observations implicate SirT3 in the regulation of mitochondrial thermogenesis. First, exposure to cold temperatures increases SirT3 expression in brown adipocytes, while elevated temperatures reduce SirT3 expression (6). Second, over-expression of SirT3 results in increased levels of the mitochondrial uncoupling protein 1 (UCP1) (6). SirT3 protein levels are also elevated in certain breast cancers (7). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
Western blot analysis of mitochondrial extracts from SirT3 wild-type (WT) and knockout (KO) mouse liver, and of whole cell extracts from rat liver and human kidney, using SirT3 (D22A3) Rabbit mAb (upper) and AIF (D39D2) XP® Rabbit mAb #5318 (lower). 使用SirT3 (D22A3) Rabbit mAb 兔单抗(上图) and AIF (D39D2) XP® Rabbit mAb兔单抗 #5318 (下图)，免疫印迹(Western blot)分析SirT3 wild-type (WT) 和knockout (KO) 小鼠肝脏的线粒体提取物，大鼠肝脏和人肝脏的全细胞裂解物中的蛋白水平。 |
