SirT5 (D5E11) Rabbit mAb
货号：
8779S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 8779S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SirT5 (D5E11) Rabbit mAb recognizes endogenous levels of total SirT5 protein. This antibody does not cross-react with other sirtuin proteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT5, a mammalian homolog of Sir2, is localized to the mitochondria and has been implicated in the regulation of cell metabolism (2,3). SirT5 deacetylates carbamoyl phosphate synthetase 1 (CPS1) in the mitochondrial matrix and increases its activity in response to fasting, allowing for adaptation to increased amino acid catabolism (4). SirT5 has also been shown to deacetylate cytochrome c in the mitochondrial intermembrane space (5). In addition to its deacetylase activity, SirT5 contains lysine desuccinylase and demalonylase activity (6,7). Succinyl-lysine and malonyl-lysine modifications occur in a variety of organisms and these post-translational modifications are found on many metabolic enzymes (6-8). Like phosphorylation of serine, threonine, and tyrosine residues, lysine succinylation and malonylation induces a change of two negative charges from a +1 to a -1 charge at physiological pH, and are thought to serve similar functions in the regulation of protein activity, protein-protein interactions, and protein stability. SirT5 knockout mice show increased levels of succinyl-lysine and malonyl-lysine protein modifications in the liver, including increased succinylation of CPS1, a known target of SirT5, suggesting that SirT5 functions to regulate metabolic enzymes through its deacetylase, desuccinylase, and demalonylase activities (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 30 |
参考图片Western blot analysis of extracts from 293 cells, expressing either nontargeting shRNA (293 shNT) or shRNA targeting SirT5 (293 shSirT5), and HCT 116, Raw 264.7, and COS-7 cells using SirT5 (D5E11) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). 293 shNT and 293 shSirT5 cells were kindly provided by David Lombard, University of Michigan.Western blot方法检测nontargeting shRNA (293 shNT)或 shRNA targeting SirT5 (293 shSirT5) 在293， HCT 116， Raw 264.7，和 COS-7细胞提取物的表达，使用的抗体为SirT5 (D5E11) Rabbit mAb (上图) 或β-Actin (D6A8) Rabbit mAb #8457 (下图)。293 shNT 和 293 shSirT5 细胞由 David Lombard, University of Michigan 惠赠。 |
