| 货号 | 59019S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IF-IC ChIP |
| 目标/特异性 | PAX8 (D2S2I) Rabbit mAb recognizes endogenous levels of total PAX8 protein. PAX8 (D2S2I) Rabbit mAb may detect other isoforms of Pax8. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:200) ChIP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14. 2 . Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62. 3 . Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94. 4 . Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803. 5 . Kimura, S. (2011) J Thyroid Res 2011, 710213. 6 . Fagman, H. and Nilsson, M. (2011) J Mol Endocrinol 46, R33-42. 7 . Albarel, F. et al. (2012) Ann Endocrinol (Paris) 73, 155-64. |
Confocal immunofluorescent analysis of OVCAR3 (left) and HeLa (right) cells using PAX8 (D2S2I) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Western blot analysis of extracts from ACHN, OVCAR3, and IGROV-1 cells using PAX8 (D2S2I) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 786-O cells and either 10 μl of PAX8 (D2S2I) Rabbit mAb #59019, or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by Real-Time PCR using SimpleChIP®Human CTGF Promoter Primers #14927, human PAX8 promoter primers, and SimpleChIP®Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
