| 货号 | 3122S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Smad2 (86F7) Rabbit mAb detects endogenous levels of total Smad2 protein. No cross reactivity was detected with Smad3. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TGF-β treated (right), using Smad2 (86F7) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red)。 使用Smad2 (86F7) Rabbit mAb兔单抗（绿色）对未处理（左）或TGF-β 处理（右）的HeLa细胞进行激光共聚焦荧光分析。肌动蛋白丝用Alexa Fluor® 555 phalloidin（红色）标记。 | |
