| 货号 | 12024S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Smurf2 (D8B8) Rabbit mAb recognizes endogenous levels of total Smurf2 protein. This antibody also cross-reacts with proteins of unknown origin at 250 and 46 kDa in some cell lines. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Smad ubiquitin regulatory factor 2 (Smurf2) is a HECT domain E3 ubiquitin ligase. It was initially identified as an inhibitor of TGF-β/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (1-3). Subsequent studies have revealed its role in neuronal and planar cell polarity, as well as in the senescence response and suppression of tumorigenesis (4-8). Smurf2 has a broad range of substrates including RUNX2, AMSH, Rap1B, and RNF11 (5,9-11). Smurf2 is widely expressed in various tissues. The C2 domain of Smurf2 inhibits its catalytic activity by interacting with the HECT domain (12). Research studies have shown that Smurf2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (13). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from U-2 OS and ACHN cells using Smurf2 (D8B8) Rabbit mAb. 使用Smurf2 (D8B8)Rabbit mAb对U-2OS和ACHN细胞提取物进行western blot分析。 |
