| 货号 | 62184S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | W ChIP |
| 目标/特异性 | CBFβ (D4N2N) Rabbit mAb recognizes endogenous levels of total CBFβ protein. |
| 使用方法 | WB(1:1000) ChIP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Core-binding factor subunit β (CBFβ) is a non-DNA binding transcription factor subunit that associates with and regulates the DNA binding activity of RUNX1, RUNX2, and RUNX3 (1). CBFβ is ubiquitously expressed and has been implicated in a variety of developmental processes including hematapoiesis, T cell development, chondrogenesis, and bone formation (2-7). In addition, investigators have identified CBFβ as one of the most frequently translocated genes in leukemia (8) and research studies have found it to be required for HIV immune evasion (9,10). CBFβ interacts with the viral protein VIF and triggers assembly of a ubiquitin ligase complex that targets the retroviral inhibitor APOBEC3G for degradation (9,10). |
| 存放说明 | -20C |
| 计算分子量 | 22 |
| 参考文献 | 1 . Ogawa, E. et al. (1993) Virology 194, 314-31. 2 . Wang, Q. et al. (1996) Cell 87, 697-708. 3 . Talebian, L. et al. (2007) Blood 109, 11-21. 4 . Johnson, K. et al. (2012) Science 336, 717-21. 5 . Yoshida, C.A. et al. (2002) Nat Genet 32, 633-8. 6 . Miller, J. et al. (2002) Nat Genet 32, 645-9. 7 . Kundu, M. et al. (2002) Nat Genet 32, 639-44. 8 . Goyama, S. and Mulloy, J.C. (2011) Int J Hematol 94, 126-33. 9 . Jäger, S. et al. (2012) Nature 481, 371-5. 10 . Zhang, W. et al. (2012) Nature 481, 376-9. |
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing Myc-tagged full-length human CBFβ (hCBFβ-Myc, +), using CBFβ (D4N2N) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using CBFβ (D4N2N) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 L8057 cells and either 10 μl of CBFβ (D4N2N) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Sla2 Promoter Primers #29965, mouse Sav1 promoter primers, and SimpleChIP® Mouse GAPDH Intron 2 Primers #8986. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
