| 货号 | 9389S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/F/ChIP |
| 目标/特异性 | SP1 (D4C3) Rabbit mAb recognizes endogenous levels of total SP1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:2000) F (1:100) ChIP (1:100) |
| 供应商 | CST |
| 背景 | Specificity protein 1 (SP1) is a ubiquitously expressed transcription factor belonging to the family of C2H2-type zinc finger containing DNA-binding proteins. SP1 binds GC-rich motifs with high affinity and regulates the expression of numerous mammalian genes (1,2). It interacts with many other transcription factors, such as c-Myc, Egr1, and Stat1, and with basal transcription machinery components. SP1 interacts with chromatin-modifying factors such as histone deacetylases (HDACs) and p300 in chromatin remodeling. Transcriptional activity and stability of SP1 are regulated by post-translational modification, including phosphorylation, acetylation, ubiquitylation, and glycosylation (3). Glycosylation of SP1 following insulin treatment leads to increased nuclear localization, while glucagon treatment increases cytoplasmic SP1 levels (4-6). Investigators have found high levels of SP1 in patients with Alzheimers disease (7). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SP1 (D4C3) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组化分析人源结肠癌癌组织的石蜡切片。使用SP1 (D4C3) Rabbit mAb兔单抗，对照肽段(左图)或抗原特异性肽段 (右图)。 | |
Western blot analysis of extracts from various cell lines using SP1 (D4C3) Rabbit mAb. 使用SP1 (D4C3) Rabbit mAb兔单抗，免疫印迹(Western blot)分析不同细胞中SP1 (D4C3)蛋白水平。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells and either 5 μl of SP1 (D4C3) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP® Human DHFR Intron 1 Primers #7531, human TIN2 intron 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 使用SimpleChIP®Enzymatic Chromatin IP Kit (Magnetic Beads) #9003，来自4 x 106 293细胞的交联染色质以及5 µl SP1 (D4C3) Rabbit mAb或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀实验。使用SimpleChIP® Human DHFR Intron 1 Primers #7531、human TIN2 intron 1 primers和SimpleChIP® Human α Satellite Repeat Primers #4486，浓缩的DNA通过real-time PCR定量。在每个样品中免疫沉淀DNA的数量被当做一个相对于1的总input chromatin数量的信号。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using SP1 (D4C3) Rabbit mAb. 使用SP1 (D4C3) Rabbit mAb兔单抗，免疫组化分析人源肺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using SP1 (D4C3) Rabbit mAb. 使用SP1 (D4C3) Rabbit mAb兔单抗，免疫组化分析人源前列腺癌组织石蜡切片。 | |
Flow cytometric analysis of HeLa cells using SP1 (D4C3) Rabbit mAb #9389 (blue) compared to concentration matched Rabbit (DA1E) mAb XP® Isotype Control #3900 (red). Anti-Rabbit IgG (H+L), (F(ab)2 Fragment (Alexa Fluor 488 Conjugate) #4412 was used as a secondary antibody |
