| 货号 | 12071S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | SPHK1 (D1H1L) Rabbit mAb recognizes endogenous levels of total SPHK1 protein. This antibody also cross-reacts with a protein of unknown origin at 160 kDa in some cell lines. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Together with other sphingolipid metabolizing enzymes, SPHKs regulate the balance of the lipid mediators, ceramide, sphingosine, and S1P (1-4). Two distinct SPHK isoforms, SPHK1 and SPHK2, have been cloned and characterized (5,6). SPHK1 and SPHK2 are highly conserved and diversely expressed (7,8). The SPHKs are activated by G protein-coupled receptors, receptor tyrosine kinases, immunoglobulin receptors, cytokines, and other stimuli (9-12). The molecular mechanisms by which SPHK1 and SPHK2 are specifically regulated are complex and only partially understood. |
| 存放说明 | -20C |
| 计算分子量 | 45-60 |
Western blot analysis of extracts from various cell lines using SPHK1 (D1H1L) Rabbit mAb. Western blot方法检测多个细胞系的提取物，使用的抗体为SPHK1 (D1H1L) Rabbit mAb. | |
Immunoprecipitation of SPHK1 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SPHK1 (D1H1L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SPHK1 (D1H1L) Rabbit mAb. 免疫沉淀法检测来自HeLa细胞提取物的SPHK1，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道 2) 或SPHK1 (D1H1L) Rabbit mAb (泳道3). 泳道1是加入10%蛋白。进行western blot方法检测，使用的抗体为SPHK1 (D1H1L) Rabbit mAb. |
