| 货号 | 14136S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:800) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Spinophilin is an 815 amino acid protein composed of a PDZ domain, 2 actin-binding domains, a receptor- and PP1-binding domain, three coiled-coiled domains, a potential leucine/isoleucine zipper motif, and three potential SH3 domains (1). Spinophilin interacts with a large number of proteins including ion channel components and G protein-coupled receptors (GPCRs). Spinophilin also interacts with actin filaments; phosphorylation of spinophilin at Ser94 and Ser177 disrupts this interaction (2). Spinophilin has been shown to affect GPCR function through two different mechanisms: spinophilin acts as a functional inhibitor of α-2 adrenergic receptor-mediated arrestin signaling by competing with GRK2 binding to the adrenergic receptor (3) and spinophilin facilitates μ-opioid receptor desensitization by promoting receptor endocytosis (4). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Immunohistochemical analysis of paraffin-embedded mouse cerebellum using Spinophilin (E1E7R) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded rat brain using Spinophilin (E1E7R) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Spinophilin (E1E7R) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from various tissues and cell lines using Spinophilin (E1E7R) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of spinophilin from mouse brain extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Spinophilin (E1E7R) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Spinophilin (E1E7R) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded MCF7 (left) and Huh7 (right) cell pellets using Spinophilin (E1E7R) Rabbit mAb. |
