| 货号 | 7695S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | IP/IF-IC |
| 目标/特异性 | SQSTM1/p62 (D10E10) Rabbit mAb (IF Preferred) is recommended to detect endogenous levels of total SQSTM1/p62 protein by immunofluorescence. Products SQSTM1/p62 (D5E2) Rabbit mAb #8025 and SQSTM1/p62 Antibody #5114 are preferred for western blot. |
| 使用方法 | IP(1:50) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress, and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5), and independently found to interact with PKCζ (6,7). It was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4,10,11). SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity. |
| 存放说明 | -20C |
| 计算分子量 | 62 |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with bafilomycin A (100 nM, 18 hr; right), using SQSTM1/p62 (D10E10) Rabbit mAb (IF Preferred) (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光试验分析HeLa细胞，未处理（左图）或洛霉素A（100 nM, 18 小时，右图），使用抗体是 SQSTM1/p62 (D10E10) Rabbit mAb 兔单抗（IF首选） (绿色)。肌动蛋白微丝使用 DY-554 phalloidin标记（红色）。蓝色伪彩=DRAQ5® #4084（荧光DNA染料）。 |
