| 货号 | 21792S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/ChIP |
| 使用方法 | WB(1:1000) IP (1:50) ChIP (1:50) |
| 供应商 | CST |
| 背景 | ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9). |
| 存放说明 | -20C |
| 计算分子量 | Iso1 60, Iso2 50 |
Western blot analysis of extracts from various cell lines using SS18 (D6I4Z) Rabbit mAb. | |
Immunoprecipitation of SS18 from ACHN cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype control #3900, and lane 3 is SS18 (D6I4Z) Rabbit mAb. Western blot analysis was performed using SS18 (D6I4Z) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d then treated with β-estradiol (10 nM) for 45 min and either 10 μl of SS18 (D6I4Z) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
