| 货号 | 13578S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | <p>Cofilin is an evolutionarily conserved, actin-binding protein that severs actin filaments during processes that rely on actin filament dynamics, including cytokinesis, cell migration, invasion, and neuronal development. Actin severing and filament depolymerization are regulated through the controlled cycling of cofilin between the phosphorylated and dephosphorylated forms (1). The kinases LIMK and TESK inactivate cofilin by phosphorylating it at Ser3 (2,3). The slingshot homologs (SSH1, SSH2 and SSH3) and chronophin/PDXP phosphatases remove phosphate from cofilin at Ser3, enabling cofilin binding to actin and filament depolymerization (3). LIMK and SSH1 regulate cofilin activity downstream of neuregulin signaling in Schwann cells (4).</p><p>Slingshot homolog 1 (SSH1) can also dephosphorylate LIMK kinases, suppressing LIMK phosphorylation of cofilin (5). In addition, SSH1 modulates actin dynamics by stabilizing F-actin and promoting actin bundling independent of its cofilin phosphatase activity (6). SSH1 activity is regulated by phosphorylation and protein-protein interaction through various signaling pathways (1). Binding of SSH1 to F-actin stimulates its cofilin phosphatase activity (7).</p> Fofilin是一个在进化上保守的actin结合蛋白，可以在需要肌动蛋白丝变化的时候切断肌动蛋白丝，包括胞质分裂，细胞迁移，侵袭和神经元发育。肌动蛋白丝的切断和中间丝解聚是通过cofilin的磷酸化和非磷酸化状态转换来调控的(1).激酶LIMK和TESK能够磷酸化cofilin的Ser3而失活cofilin（2,3）。Slingshot homolog（SSH1, SSH2和 SSH3）和chronophin/PDXP磷酸酶将cofilin的Ser3的磷酸基团移除，使cofilin可以和肌动蛋白丝结合以及参与中间丝的解聚（3）。LIMK和 SSH1在Schwann细胞中调控neuregulin信号下游的cofilin活性（4）。lingshot homolog 1 (SSH1)可以去磷酸化LIMK激酶，抑制LIMK磷酸化cofilin(5).除此以外，SSH1通过稳定F-actin调控肌动蛋白丝的运动，并且在不依赖cofilin磷酸酶活性的条件下促进肌动蛋白丝集合成束（6）。SSH1的活性是通过磷酸化和多种信号通路的蛋白与蛋白相互作用来调控的（1）。SSH1和F-actin的结合激活了SSH1的磷酸酶活性（7）。 |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Western blot analysis of extracts from various cell lines using SSH1 (E1K3W) Rabbit mAb.使用SSH1 (E1K3W) Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human SSH1 protein (hSSH1-Myc/DDK; +), using SSH1 (E1K3W) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).模拟转染（-）或转让了表达Myc/DDK-tagged全长人SSH1 (hSSH1-Myc/DDK; +)的293T细胞提取物，使用SSH1 (E1K3W) Rabbit mAb (上) 或Myc-Tag (71D10) Rabbit mAb #2278 (下)进行western blot分析。 | |
Immunoprecipitation of SSH1 from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SSH1 (E1K3W) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using SSH1 (E1K3W) Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或 SSH1 (E1K3W) Rabbit mAb (lane 3)对Jurkat细胞提取物中的SSH1进行免疫沉淀实验。Lane1是10%上样量。使用SSH1 (E1K3W) Rabbit mAb进行western blot分析。 |
