| 货号 | 12816S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | SSU72 (D3I2D) Rabbit mAb recognizes endogenous levels of total SSU72 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | SSU72 is a protein phosphatase originally identified through its interaction with TFIIB as a factor required for transcription and RNA processing (1,2). While SSU72 has been known as a phosphatase of the RNA polymerase II carboxy-terminal domain at Ser5 of the heptapeptide repeat, additional studies suggest a role for SSU72 as a phosphatase for Ser7 of the heptapeptide repeat as well (3-9). The activities of SSU72 are thought to play a critical role in coupling transcription with pre-mRNA 3’ end processing (10-12). SSU72 is required for gene looping that connects the gene promoter with the terminator region, leading to proper initiation of transcription and enforcement of transcriptional directionality on promoters that may other wise act as bidirectional promoters (13-14). In addition to a role in transcription, SSU72 acts as a cohesin binding protein that regulates cohesion between sister chromatid arms during mitosis or meiosis (15). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
Western blot analysis of extracts from various cell lines using SSU72 (D3I2D) Rabbit mAb.Western blot方法检测不同细胞系的提取物，使用的抗体为 SSU72 (D3I2D) Rabbit mAb。 | |
Immunoprecipitation of SSU72 from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SSU72 (D3I2D) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SSU72 (D3I2D) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.从 293T细胞提取物中免疫沉淀SSU72，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或 SSU72 (D3I2D) Rabbit mAb (lane 3)。Lane 1为10% input。 使用SSU72 (D3I2D) Rabbit mAb进行Western blot检测，二抗使用的是Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678。 |
