| 货号 | 9175S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | Stat1 (42H3) Rabbit mAb detects endogenous levels of total Stat1 protein independent of phosphorylation. |
| 使用方法 | WB(1:1000) IHC-P (1:400) |
| 供应商 | CST |
| 背景 | The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. |
| 存放说明 | -20C |
| 计算分子量 | 84, 91 |
Immunohistochemical analysis of paraffin-embedded human MALToma, showing cytoplasmic and nuclear staining, using Stat1 (42H3) Rabbit mAb (Human Specific).免疫组织化学染色分析石蜡包埋人粘膜相关淋巴样组织淋巴瘤，图所示为细胞质和细胞核的染色。所用抗体为Stat1 (42H3) Rabbit mAb (人源特异性)。 | |
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkins lymphoma, using Stat1 (42H3) Rabbit mAb (Human Specific).免疫组织化学染色分析石蜡包埋人非霍奇金淋巴瘤。所用抗体为Stat1 (42H3) Rabbit mAb (人源特异性)。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Stat1 (42H3) Rabbit mAb (Human Specific) in the presence of control peptide (left) or Stat1 blocking peptide (9175 specific) #1079 (right).免疫组织化学染色分析石蜡包埋人结肠癌组织。在对照多肽(左图)或者Stat1 封闭多肽 (9175特异性) #1079 (右图)存在下所用抗体为Stat1 (42H3) Rabbit mAb (人源特异性) 。 | |
Western blot analysis of extracts from HeLa and COS cells, using Stat1 (42H3) Rabbit mAb (Human Specific).Western免疫印迹。用Stat1 (42H3) Rabbit mAb (人源特异性)研究HeLa 和 COS 细胞的细胞提取液。 |
