| 货号 | 9176S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IP |
| 目标/特异性 | Stat1 (9H2) Mouse mAb detects endogenous levels of total Stat1 protein independent of phosphorylation, however, it prefers the non-phosphorylated form of Stat1. The antibody detects both Stat1alpha (91 kDa) and Stat1beta (84 kDa) isoforms. It does not significantly cross-react with the other Stat proteins. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. |
| 存放说明 | -20C |
| 计算分子量 | 84, 91 |
Western blot analysis of extracts from HeLa cells, untreated or IFN-alpha-treated (100 ng/ml for 5 minutes), using Stat1 (9H2) Mouse mAb.Western免疫印迹。用 Stat1 (9H2) Mouse mAb 抗体研究未经处理的和经IFN-alpha(100 ng/ml,5 min)处理的 HeLa 细胞的细胞提取液。 | |
Immunoprecipitation of extracts from HeLa cells, untreated or IFN-alpha-treated (100 ng/ml for 5 minutes), using Stat1 (9H2) Mouse mAb, followed by Western blotting with Phospho-Stat1 (Tyr701) Antibody #9171.免疫共沉淀未经处理的和经IFN-alpha(100 ng/ml,5 min)处理的HeLa细胞的细胞提取液，所用抗体为Stat1 (9H2) Mouse mAb,然后用Phospho-Stat1 (Tyr701)抗体 #9171做Western免疫印迹实验。 |
