| 货号 | 9139S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P/IF-IC/F/ChIP |
| 目标/特异性 | Stat3 (124H6) Mouse mAb detects endogenous levels of total Stat3 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:600) F (1:200) IF-IC (1:1600) ChIP (1:100) |
| 供应商 | CST |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | -20C |
| 计算分子量 | 79, 86 |
Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using Stat3 (124H6) Mouse mAb.Western免疫印迹。用Stat3 (124H6) Mouse mAb研究HeLa, NIH/3T3, PC12 和 COS细胞的细胞提取液。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left), showing nuclear and cytoplasmic staining, and human lung carcinoma (right), showing cytoplasmic staining, using Stat3 (124H6) Mouse mAb.免疫组织化学染色分析石蜡包埋人乳腺癌组织(左图),图片显示了细胞核和细胞质的染色和石蜡包埋人肺癌组织(右图),图片显示了细胞质的染色。所用抗体为Stat3 (124H6) Mouse mAb。 | |
Flow cytometric analysis of Hela cells, untreated (blue) or IFN-alpha-treated (green), using Stat3 (124H6) Mouse mAb compared with a nonspecific negative control antibody (red).流式细胞仪研究未经处理的HeLa细胞(蓝色)和经IFN-alpha处理的HeLa细胞(绿色)。所用抗体为Stat3 (124H6) Mouse mAb 和非特异性阴性对照抗体(红色)。 | |
Confocal immunofluorescent analysis of HeLa cells either serum-starved (left) or IFNalpha-treated (right) and labeled with Stat3 (124H6) Mouse mAb (green).共聚焦免疫荧光分析经血清饥饿的HeLa(左图)或者IFNalpha处理的(右图) HeLa,用Stat3 (124H6) Mouse mAb (绿色)标记。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hep G2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes, and either 10 μl of Stat3 (124H6) Mouse mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。Hep G2 细胞培养至4 x 106, 并经过夜饥饿或IL-6(100 ng/ml,30 min) 处理,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用10 μl Stat3 (124H6) Mouse mAb 或2 μl Normal Rabbit IgG #2729 抗体。用人源 IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。 |
