| 货号 | 4904S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | Stat3 (79D7) Rabbit mAb detects endogenous levels of total Stat3 protein. |
| 使用方法 | WB(1:2000) IP (1:100) ChIP (1:50) |
| 供应商 | CST |
| 背景 | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| 存放说明 | -20C |
| 计算分子量 | 79, 86 |
Western blot analysis of extracts from various cell lines using Stat3 (79D7) Rabbit mAb.Western免疫印迹。用Stat3 (79D7) Rabbit mAb 研究各种细胞的细胞提取液。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Stat3 siRNA I (+) or SignalSilence® Stat3 siRNA II #6582 (+), using Stat3 (79D7) Rabbit mAb #4904 and α-Tubulin (11H10) Rabbit mAb #2125. The Stat3 (79D7) Rabbit mAb confirms silencing of Stat3 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Stat3 siRNA.Western免疫印迹。用Stat3 (79D7) Rabbit mAb #4904 或α-Tubulin (11H10) Rabbit mAb #2125 研究转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或SignalSilence® Stat3 siRNA I (+) 或 SignalSilence® Stat3 siRNA II #6582 (+) 的HeLa细胞的细胞提取液。Stat3 (79D7) Rabbit mAb 证实了对Jak3 蛋白表达的沉默, α-Tubulin (11H10) Rabbit mAb作为上样量的对照和Jak3的特异性。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Hep G2 cells treated with IL-6 (100 ng/ml) for 30 minutes, and either 20 μl of Stat3 (79D7) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。 Hep G2 细胞培养至4 x 106， 并经IL-6(100 ng/ml)处理30分钟后, 然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用20 μl of Stat3 (79D7) Rabbit mAb 抗体 或2 μl Normal Rabbit IgG #2729 。用human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663和SimpleChIP® Human α Satellite Repeat 引物 #4486 对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。 |
