| 货号 | 2653S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/ChIP |
| 目标/特异性 | Stat4 (C46B10) Rabbit mAb detects endogenous levels of total Stat4 protein. |
| 使用方法 | WB(1:1000) ChIP (1:50) |
| 供应商 | CST |
| 背景 | The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins resulting in their nuclear translocation, binding to specific DNA elements and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8). |
| 存放说明 | -20C |
| 计算分子量 | 81 |
Western blot analysis of extracts from ACHN, SR and Caki cell lines and from rat thymus using Stat4 (C46B10) Rabbit mAb.Western免疫印迹。用Stat4 (C46B10) Rabbit mAb研究ACHN, SR 和Caki 细胞系的细胞提取液以及大鼠胸腺提取液。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NK-92 cells starved of IL-2 overnight then treated with IL-12 (10ng/ml) for 4h and either 20 μl of Stat4 (C46B10) Rabbit mAb #2653 or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, human PRF1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。NK-92细胞培养至4 x 106，IL-2饥饿处理过夜后经IL-12(10ng/ml)诱导4小时,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用20 μlStat4 (C46B10) Rabbit mAb #2653抗体或2μl Normal Rabbit IgG #2729 。用human IRF-1 promoter primers, human PRF1 promoter primers和SimpleChIP® Human α Satellite Repeat Primers #4486对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。 | |
Western blot analysis of extracts from various cell lines and from rat thymus using Stat4 (C46B10) Rabbit mAb. |
