| 货号 | 44464S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IHC |
| 目标/特异性 | CEACAM1 (D3R8O) Rabbit mAb recognizes endogenous levels of total CEACAM1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunohistochemistry (Paraffin) (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CEACAM1 (also known as C-CAM and CD66a) is a member of CEA-related cell-adhesion molecule (CEACAM) subfamily of the carcinoembryonic antigen (CEA) family (1). CEACAM1 is expressed by certain epithelial, endothelial, lymphoid, and myeloid cells. Human CEACAM1 has many different splice variants; the abundance of CEACAM1 and the relative ratio of the different isoforms varies markedly among cell types and may be regulated in a context-dependent fashion. The isoforms with long (L) and short (S) cytoplasmic tails have different signaling properties. Notably, L isoforms contain a functional ITIM (immunoreceptor tyrosine-based inhibitory motif) and several serine and threonine residues that could serve as potential phosphorylation targets. The extracellular domain of CEACAM1 is heavily glycosylated, making its apparent molecular weight during electrophoresis much larger than its predicted size (57.6 kDa) (2). CEACAM1 mediates intercellular adhesion through homo- and heterophilic interaction with other members of the CEACAM family. Studies indicate that CEACAM1 plays important roles in angiogenesis, neovascularization, insulin signaling, T cell signaling, and tumorigenesis (3-8). In addition, CEACAM1 can function as a receptor for several microbial pathogens (9,10). |
| 存放说明 | -20C |
| 计算分子量 | 120-150 |
| 参考文献 | 1 . Kuespert, K. et al. (2006) Curr Opin Cell Biol 18, 565-71. 2 . Gray-Owen, S.D. and Blumberg, R.S. (2006) Nat Rev Immunol 6, 433-46. 3 . Horst, A.K. et al. (2006) J Clin Invest 116, 1596-605. 4 . Ergün, S. et al. (2000) Mol Cell 5, 311-20. 5 . Kammerer, R. et al. (2001) J Immunol 166, 6537-44. 6 . Poy, M.N. et al. (2002) Nat Genet 30, 270-6. 7 . Hsieh, J.T. et al. (1995) Cancer Res 55, 190-7. 8 . Leung, N. et al. (2006) Oncogene 25, 5527-36. 9 . Hemmila, E. et al. (2004) J Virol 78, 10156-65. 10 . Voges, M. et al. (2010) BMC Microbiol 10, 117. |
Western blot analysis of extracts from Hep G2, SK-MEL-5, and HeLa cell lines using CEACAM1 (D3R8O) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CEACAM1 (D3R8O) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CEACAM1 (D3R8O) Rabbit mAb. |
