| 货号 | 13655S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 使用方法 | WB(1:1000) IHC-P (1:2000) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Stathmin is a ubiquitously expressed microtubule destabilizing phosphoprotein that is upregulated in a number of cancers. The amino terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization. Phosphorylation at these sites inactivates the protein and stabilizes microtubules. Ser16 phosphorylation by CaM kinases II and IV (1,2) increases during G2/M-phase and is involved in mitotic spindle regulation (3,4). Ser38 is a target for cdc2 kinase (5) and TNF-induced cell death gives rise to reactive oxygen intermediates leading to hyperphosphorylation of stathmin (6). EGF receptor activation of Rac and cdc42 also increases phosphorylation of stathmin on Ser16 and Ser38 (7). Other closely related family members are neuronally expressed and include SCG10, SCLIP, RB3 and its splice variants RB3 and RB3. Stathmin and SCG10 have been shown to play roles in neuronal-like development in PC-12 cells (8). |
| 存放说明 | -20C |
| 计算分子量 | 19 |
Confocal immunofluorescent analysis of C2C12 cells using Stathmin (D1Y5A) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded mouse kidney using Stathmin (D1Y5A) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Stathmin (D1Y5A) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Stathmin (D1Y5A) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Stathmin (D1Y5A) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lymph node using Stathmin (D1Y5A) Rabbit mAb. |
