| 货号 | 2802S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Mouse |
| 应用 | W |
| 目标/特异性 | Survivin (6E4) Mouse mAb detects endogenous levels of human survivin. The antibody does not cross-react with other types of apoptotic inhibitor proteins. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 16 |
Western blot analysis of extracts from 293 and HeLa cells, using Survivin (6E4) Mouse mAb. Western blot检测293和HeLa 细胞提取物，使用抗体为 Survivin (6E4) Mouse mAb 鼠单抗。 | |
Western blot analysis of extracts from HeLa cells 48 hours following mock transfection, transfection with nonspecific (control) siRNA or transfection with Survivin siRNA. Survivin was detected using Survivin (6E4) Monoclonal Antibody #2802 and p42 was detected using p42 MAPK Antibody #9108. The Survivin Antibody confirms silencing of Survivin expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity. Western blot方法检测HeLa细胞提取物，48h空质粒、非特异性（对照）siRNA 或Survivin siRNA转染；使用Survivin (6E4) Monoclonal Antibody #2802检测Survivin表达，或用p42 MAPK Antibody #9108检测p42表达。Survivin Antibody确定Survivin表达的沉默，p42 MAPK作为内参对照。 |
