| 货号 | 2808S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC/F |
| 目标/特异性 | Survivin (71G4B7E) Rabbit mAb detects endogenous levels of total Survivin protein. |
| 使用方法 | WB(1:1000) IP (1:400) IHC-P (1:400) F (1:400) IF-IC (1:1000) IHC-F (1:400) |
| 供应商 | CST |
| 背景 | Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 16 |
Western blot analysis of lysates from Raji (human), BaF3 (mouse) and C6 (rat) cell lines using Survivin (71G4B7E) Rabbit mAb. Western blot检测Raji (人)、BaF3 (小鼠)和C6 (大鼠) 细胞系提取物，使用抗体为Survivin (71G4B7E) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Survivin (71G4B7E) Rabbit mAb in the presence of control peptide (left) or Survivin Blocking Peptide #1037 (right). 免疫组织化学分析石蜡包埋的人结肠癌切片，用对照肽（左）或 Survivin Blocking Peptide #1037 （右），使用抗体为 Survivin (71G4B7E) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing nuclear localization using Survivin (71G4B7E) Rabbit mAb #2808. 免疫组织化学分析石蜡包埋的人肺癌细胞，使用Survivin (71G4B7E) Rabbit mAb 兔单抗#2808 进行细胞核定位。 | |
Immunohistochemical analysis of paraffin-embedded human pituitary adenoma using Survivin (71G4B7E) Rabbit mAb. 免疫组织化学分析石蜡包埋的人垂体瘤切片，使用抗体为 Survivin (71G4B7E) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder using Survivin (71G4B7E) Rabbit mAb. 免疫组织化学分析石蜡包埋的人膀胱移行上皮癌切片，使用抗体为 Survivin (71G4B7E) Rabbit mAb 兔单抗。 | |
Flow cytometric analysis of untreated Jurkat cells using Survivin (71G4B7E) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 流式细胞术检测未处理的Jurkat细胞，使用抗体为Survivin (71G4B7E) Rabbit mAb 兔单抗（蓝色），一个非特异性阴性对照抗体（红色）作为对照。 | |
Confocal immunofluorescent analysis of HeLa cells using Survivin (71G4B7E) Rabbit mAb (green). Mitochondria have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa细胞，使用抗体为Survivin (71G4B7E) Rabbit mAb 兔单抗（绿色）。MitoTracker® Red CMXRos （红色）进行线粒体共定位。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Immunohistochemical analysis of frozen H1650 xenograft showing nuclear localization using Survivin (71G4B7E) Rabbit mAb. 免疫组化分析冰冻H1650异种移植物核定位，使用抗体为Survivin (71G4B7E) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Survivin siRNA II (+), using Survivin (71G4B7E) Rabbit mAb #2808 and α-Tubulin (11H10) Rabbit mAb #2125. Survivin (71G4B7E) Rabbit mAb confirms silencing of survivin expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of survivin siRNA. Western blot方法检测HeLa细胞提取物，使用100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-)或SignalSilence® Survivin siRNA II (+)转染；使用Survivin (71G4B7E) Rabbit mAb 兔单抗#2808或α-Tubulin (11H10) Rabbit mAb 兔单抗#2125。Survivin (71G4B7E) Rabbit mAb兔单抗确定Survivin表达的沉默，α-Tubulin (11H10) Rabbit mAb 兔单抗作为内参对照。 |
