货号 | 85852S |
反应种属 | Mouse/Rat |
来源宿主 | Rabbit IgG |
应用 | W/IP/IF-F |
使用方法 | WB(1:1000) IP (1:50) IF-F (1:400) |
供应商 | CST |
背景 | Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb, and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of the synapsin amino-terminal domain at Ser9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2). |
存放说明 | -20C |
计算分子量 | 77 |
Confocal immunofluorescent analysis of normal mouse hippocampus (left), cortex (middle), and cerebellum (right) using Synapsin-2 (D6S9C) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (Fluorescent DNA dye). | |
Western blot analysis of extracts from mouse brain, mouse lung, and rat brain tissues using Synapsin-2 (D6S9C) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of synapsin-2 from Neuro-2a cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Synapsin-2 (D6S9C) Rabbit mAb. Western blot analysis was performed using Synapsin-2 (D6S9C) Rabbit mAb. |