| 货号 | 71632S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W |
| 目标/特异性 | Tristetraprolin (D1I3T) Rabbit mAb recognizes endogenous levels of total Trisetraprolin protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tristetraprolin (TTP), also known as NUP475, G0S24, RNF162A, TIS11, and ZFP36, is a CCCH tandem zinc-finger protein that binds to adenosine and uridine (AU)-rich elements (AREs) within 3-untranslated regions of mRNA and leads to their rapid degradation (1-6). Expression of TTP is rapidly induced by mitogens and growth factors including insulin, phorbol ester, cytokines, and lipopolysaccharide (LPS). In addition, numerous phosphorylation sites on TTP can regulate its stability, nuclear to cytosolic trafficking, as well as controlling its ARE-binding activity. Many of the target mRNAs for TTP, such as TNF-α, have critical roles in inflammation and cancer (2), and mice deficient in TTP develop a systemic autoimmune inflammatory syndrome along with excessive TNF-α levels (7). Furthermore, suppression of TTP expression has been identified as a negative prognostic indicator for some cancers (8). |
| 存放说明 | -20C |
| 计算分子量 | 40-48 |
| 参考文献 | 1 . Brooks, S.A. and Blackshear, P.J. Biochim Biophys Acta 1829, 666-79. 2 . Sanduja, S. et al. (2012) Front Biosci (Landmark Ed) 17, 174-88. 3 . Lai, W.S. et al. (1990) J Biol Chem 265, 16556-63. 4 . DuBois, R.N. et al. (1990) J Biol Chem 265, 19185-91. 5 . Varnum, B.C. et al. (1989) Oncogene 4, 119-20. 6 . Heximer, S.P. and Forsdyke, D.R. DNA Cell Biol 12, 73-88. 7 . Taylor, G.A. et al. (1996) Immunity 4, 445-54. 8 . Brennan, S.E. et al. (2009) Cancer Res 69, 5168-76. |
Western blot analysis of extracts from Raw 264.7 cells treated with Lipopolysaccharides (LPS) #14011 (1 μg/ml, 4 hr), with (+) or without (-) treatment with phophatases, Calf Intestinal Phophatase (CIP) and λ-Phosphatase (λ-Phosphatase). | |
Western blot analysis of extracts from THP-1 cells, differentiated with TPA #4174 (80 nM, 24 hr) followed by treatment with Lipopolysaccharides (LPS) #14011 (1 μg/ml, 6 hr; +) or without additional treatment (-) using Tristetraprolin (D1I3T) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from various cell lines using Tristetraprolin (D1I3T) Rabbit mAb. KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge. | |
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated with Lipopolysaccharides (LPS) #14011 (1 μg/ml, 4 hr; +) using Tristetraprolin (D1I3T) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
