| 货号 | 12578S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | TBP (D5G7Y) Rabbit mAb recognizes endogenous levels of total TBP protein. |
| 使用方法 | WB(1:1000) IP (1:50) ChIP (1:50) |
| 供应商 | CST |
| 背景 | TATA-binding protein (TBP) is a ubiquitously expressed nuclear protein that functions at the core of the general transcription factor protein complex TFIID (1-3). TFIID, which contains TBP and 13 TBP-associated factors (TAFs), contributes to the formation of the transcription pre-initiation complex, an assembly of multiple protein complexes (TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II) that bind to a gene promoter during the initiation of transcription (1-3). Once the pre-initiation complex is formed, RNA polymerase II becomes competent for elongation and transcribes the body of a gene. TBP functions in the recruitment of TFIID by binding to the TATA-box sequence found approximately 25 base pairs upstream of the transcription start site of many protein-coding genes. In addition, many transcriptional activator proteins interact with TBP and various TAF proteins to facilitate recruitment of TFIID and formation of the pre-initiation complex. |
| 存放说明 | -20C |
| 计算分子量 | 38 |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of TBP (D5G7Y) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human γ-Actin Promoter Primers #5037, SimpleChIP® Human GAPDH Promoter Primers #4471 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 对4 x 106 HeLa细胞中交联过的染色质进行染色质免疫沉淀，所用抗体为10 µl Mono-Methyl-Histone H3 (Lys79) (D5X1S) Rabbit mAb 或2 µl Normal Rabbit IgG #2729，富集到的DNA经过实时PCR定量，所使用产品为SimpleChIP® Human γ-Actin Promoter Primers #5037， SimpleChIP® Human GAPDH Promoter Primers #4471 以及SimpleChIP® Human α Satellite Repeat Primers #4486。每个样品中免疫沉淀得到的DNA量由与input chromatin( 相当于1)的相对信号值来表示。 | |
Western blot analysis of extracts from various cell lines using TBP (D5G7Y) Rabbit mAb.Western blot方法检测多种细胞系的提取物，使用的抗体为TBP (D5G7Y) Rabbit mAb。 | |
Immunoprecipitation of TBP from HCT 116 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or TBP (D5G7Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TBP (D5G7Y) Rabbit mAb.从 HCT 116细胞提取物中免疫沉淀TBP,使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane2)或TBP (D5G7Y) Rabbit mAb (lane 3)。Lane 1为10% input。 使用TBP (D5G7Y) Rabbit mAb进行Western blot检测。 |
