| 货号 | 74499S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP ChIP |
| 目标/特异性 | TBL1XR1/TBLR1 (D4J9C) Rabbit mAb recognizes endogenous levels of total TBL1XR1/TBLR1 protein. This antibody also cross-reacts with an unidentified protein of 130 kDa. |
| 使用方法 | WB(1:1000) IP (1:100) ChIP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transducing β-like protein 1 (TBL1X/TBL1) and TBL1-related protein 1 (TBL1XR1/TBLR1) were originally identified as subunits of the co-repressor silencing mediator for retinoic and thyroid hormone receptor (SMRT) and nuclear receptor co-repressor (NCoR) complexes (1-3). These two factors are required for the exchange of co-repressor complexes for co-activators by acting as adaptors to recruit the ubiquitin/proteasome machinery that degrades the co-repressor proteins during ligand mediated activation of transcription (4,5). Co-factor exchange driven by TBL1X/TBL1 and TBL1XR1/TBLR1 appears to be the mechanism by which c-Jun and NF-κB mediated transcription is activated and is therefore likely to be the mechanism employed by other signal-dependent transcription factors as well (4,6). In addition, both TBL1X/TBL1 and TBL1XR1/TBLR1 have essential roles in regulating the Wnt-signaling pathway by recruiting β-catenin to Wnt target genes to activate transcription. Depletion of TBL1X-TBL1XR1 significantly inhibited Wnt-beta-catenin- induced gene expression and oncogenic growth in vitro and in vivo (7). Research studies have shown that upregulation of TBL1XR/TBLR1 is observed in a variety of solid tumors, and is correlated with advanced tumor stage, metastasis and poor prognosis (1). |
| 存放说明 | -20C |
| 计算分子量 | 50, 60 |
| 参考文献 | 1 . Li, J.Y. et al. (2015) Am J Clin Exp Urol 3, 13-23. 2 . Zhang, J. et al. (2002) Mol Cell 9, 611-23. 3 . Yoon, H.G. et al. (2003) EMBO J 22, 1336-46. 4 . Perissi, V. et al. (2004) Cell 116, 511-26. 5 . Perissi, V. et al. (2008) Mol Cell 29, 755-66. 6 . Hoberg, J.E. et al. (2004) Mol Cell 16, 245-55. 7 . Li, J. and Wang, C.Y. (2008) Nat Cell Biol 10, 160-9. |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and either 10 μl of TBL1XR1/TBLR1 (D4J9C) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Axin2 Intron 1 Primers #8973, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Immunoprecipitation of TBL1XR1/TBLR1 from HT-29 cell extracts. Lane 1 is 10% input. Lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 and lane 3 is TBL1XR1/TBLR1 (D4J9C) Rabbit mAb. Western blot analysis was performed using TBL1XR1/TBLR1 (D4J9C) Rabbit mAb. | |
Western blot analysis of extracts from HT-29, RPMI 8226 and COS-7 cells using TBL1XR1/TBLR1 (D4J9C) Rabbit mAb. |
