| 货号 | 2565S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | TCF4 (C9B9) Rabbit mAb detects endogenous levels of total TCF4 protein. |
| 使用方法 | WB(1:1000) IP (1:50) ChIP (1:50) |
| 供应商 | CST |
| 背景 | LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 58, 79 |
Western blot analysis of total cell lysates from HCT116, DLD1 and HepG2 cells using TCF4 (C9B9) Rabbit mAb. 使用TCF4 (C9B9)Rabbit mAb兔单抗对HCT116，DLD1和HepG2细胞全细胞提取物进行western blot分析。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and either 10 μl of TCF4 (C9B9) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 HCT 116细胞与10μl TCF4 (C9B9) Rabbit mAb 兔单抗或2μl Normal Rabbit IgG #2729进行染色质免疫共沉淀。富集到的DNA使用SimpleChIP®人CaMK2D Intron 3引物#5111，人c-Myc promoter引物和SimpleChIP®人α Satellite Repeat 引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量与input染色质总量对比进行相对定量，其中Input染色质量设定值为1。 |
