| 货号 | 5942S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Tudor domain-containing protein 3 (TDRD3) contains a tudor domain through which it binds to asymmetric di-methyl histone H3 (Arg17) and asymmetric di-methyl histone H4 (Arg3). Both of these histone marks are associated with transcription activation (1,2). TDRD3 is targeted to estrogen responsive genes where it performs as a coactivator (1). TDRD3 acts as a scaffolding protein to recruit Topoisomerase III B (TOP3B) to target genes to reduce transcription-generated R loops by relaxing negatively supercoiled DNA thereby reducing genomic instability (3). In addition to the nucleus, TDRD3 also resides in the cytoplasm where it associates with actively translating polyribosomes and accumulates into stress granules upon exposure to stress, implicating its role in post-transcriptional regulation of RNA (2,4). |
| 存放说明 | -20C |
| 计算分子量 | 83 |
Western blot analysis of extracts from various cell lines using TDRD3 (D3O2G) Rabbit mAb. | |
Immunoprecipitation of TDRD3 from SH-SY5Y cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TDRD3 (D3O2G) Rabbit mAb. Western blot analysis was performed using TDRD3 (D3O2G) Rabbit mAb. |
