| 货号 | 8097S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | TECPR1 (D6C10) Rabbit mAb recognizes endogenous levels of total TECPR1 protein. This antibody does not cross-react with TECPR2. A background band of unknown origin around 200 kDa is detected in some cell lines. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Tectonin β-propeller repeat protein (TECPR1) was first identified as DKFZP434B0335, a human ortholog of the yeast protein Pex23p (1). TECPR1 contains a pleckstrin homology (PH) domain, β-propeller domain, and dysferlin domains. Research studies have shown that elevated expression of TECPR1 may be a potential marker for prostate cancer (2). In several independent studies, TECPR1 was shown to play a role in autophagy through interaction with Atg5 (3-5). Atg5 is a protein that is conjugated to the ubiquitin-like protein Atg12 and plays an essential role in autophagy (6). Initial studies suggested that TECPR1 plays a role in selective autophagy processes by targeting bacterial pathogens, as well as damaged mitochondria and protein aggregates (4). TECPR1 appears to be important for autophagosome maturation and promotes autophagosome fusion with the lysosome (5). Deletion of TECPR1 leads to an accumulation of autophagosomes (5). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human TECPR1 (hTECPR1; +), using TECPR1 (D6C10) Rabbit mAb. Western blot分析293T细胞的细胞提取物，对照转染(-)或转染表达全长人源TECPR1的质粒(hTECPR1; +)，使用抗体是 TECPR1 (D6C10) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from various cell lines using TECPR1 (D6C10) Rabbit mAb. Western blot分析多种细胞系的细胞提取物，使用抗体是TECPR1 (D6C10) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from H-4-II-E cells, untreated (-) or treated (+) with chloroquine (50 μM, overnight), using TECPR1 (D6C10) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Western blot 分析H-4-II-E细胞的细胞提取物，未处理(-)或氯喹处理（+）（50 μM, 过夜），使用的抗体是TECPR1 (D6C10) Rabbit mAb 兔单抗(上图) 和 β-Actin (D6A8) Rabbit mAb 兔单抗#8457 (下图)。 |
