| 货号 | 3709S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | TGF-β Antibody detects recombinant TGF-β1 and TGF-β3 proteins. The antibody also detects endogenous levels of the TGF-β precursor proteins. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII) and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 12, 45-60 |
Western blot analysis of recombinant active TGF-β1 using TGF-β (56E4) Rabbit mAb.使用TGF-β (56E4)兔单抗对重组活性TGF-β1进行western blot分析。 | |
Western blot analysis of extracts of HeLa cells, mock transfected or transfected with TGF-β1 precursor, using TGF-β (56E4) Rabbit mAb.使用TGF-β (56E4)兔单抗对未转染或转染了TGF-β1前体的HeLa细胞提取物进行进行western blot分析。 | |
Western blot analysis of extracts from K-562, Saos-2 and 786-0 cells using TGF-β (56E4) Rabbit mAb.使用TGF-β (56E4) 兔单抗对K-562, Saos-2和786-0细胞提取物进行western blot分析。 |
