| 货号 | 12265S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | TH1L (D5G6W) Rabbit mAb recognizes endogenous levels of total TH1L protein. |
| 使用方法 | WB(1:1000) IP (1:100) ChIP (1:100) |
| 供应商 | CST |
| 背景 | Negative Elongation Factor (NELF) consists of four subunits: WHSC2 (NELF-A), COBRA-1 (NELF-B), TH1L (NELF-C/D), and NELF-E (1). NELF, together with DRB-sensitivity inducing factor (DSIF), inhibits RNA Polymerase II (RNAPII) elongation resulting in RNAPII promoter proximal pausing, where it awaits additional signaling to resume transcription (2,3). The release of RNAPII from promoter proximal pausing is a critical regulatory point during transcription and is signaled by positive transcription elongation factor (p-TEF-b) phosphorylation of both NELF and the C-terminal domain (CTD) within the largest subunit of RNAPII (4,5). WHSC2 is thought to connect the NELF complex to RNAPII machinery, while NELF-E contains an RNA binding motif that is necessary for NELF function (1,6,7). TH1L, together with COBRA-1, are integral subunits that bring WHSC2 and NELF-E together in the NELF complex (1). |
| 存放说明 | -20C |
| 计算分子量 | 66 |
Western blot analysis of extracts from various cell lines using TH1L (D5G6W) Rabbit mAb.Western blot方法检测不同细胞系的提取物，使用的抗体为 TH1L (D5G6W) Rabbit mAb。 | |
Immunoprecipitation of TH1L from HCT 116 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or TH1L (D5G6W) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TH1L (D5G6W) Rabbit mAb.从 HCT 116细胞提取物中免疫沉淀TH1L，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或 TH1L (D5G6W) Rabbit mAb (lane 3)。Lane 1为10% input。 使用TH1L (D5G6W) Rabbit mAb进行Western blot检测。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MCF7 cells grown in phenol red-free medium and 5% charcoal-stripped FBS for 4 d and either 5 μl of TH1L (D5G6W) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human PMAIP1 Intron 1 Primers #12558, human c-Myc intron 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用从4 x 106 HCT 116细胞中提取的交联过的染色质，以及5 µl TH1L (D5G6W) Rabbit mAb 或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀，这些细胞含5%活性炭处理胎牛血清以及无酚红的培养基中培养4天。所用试剂盒为SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。富集到的DNA经过实时PCR定量，所使用引物为SimpleChIP® Human PMAIP1 Intron 1 Primers #12558, human c-Myc intron 1 primers,以及 SimpleChIP® Human α Satellite Repeat Primers #4486。每个样品中免疫沉淀得到的DNA量由与input chromatin( 相当于1)的相对信号值来表示。 |
