| 货号 | 12655S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | THOC4/ALY (D3R4R) Rabbit mAb recognizes endogenous levels of total THOC4/ALY protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | mRNA export is a process that is tightly coupled to mRNA splicing (1-4). Splicing of mRNAs and the packaging of mRNAs in the form of mRNA-protein complex (mRNP) leads to the recruitment of the mRNA export adaptor THOC4/ALY via its interaction with the splicing factor UAP56, forming a large complex termed the transcription-export complex (TREX) (1,2,5). THOC4/ALY then directly interacts with NXF1/TAP, a part of the heterodimer that targets the mRNP to the nuclear pore complex, resulting in the shuttling of mRNP out of the nucleus and into the cytoplasm (1-3,6). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
Confocal immunofluorescent analysis of HeLa cells using THOC4/ALY (D3R4R) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 采用共聚焦免疫荧光术分别检测HeLa细胞，使用的抗体为 THOC4/ALY (D3R4R) Rabbit mAb兔单抗 (绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记（红色）。 | |
Western blot analysis of extracts from various cell lines using THOC4/ALY (D3R4R) Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为 THOC4/ALY (D3R4R) Rabbit mAb兔单抗。 | |
Immunoprecipitation of THOC4/ALY from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or THOC4/ALY (D3R4R) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using THOC4/ALY (D3R4R) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody. 免疫沉淀法检测HeLa细胞提取物中的THOC4/ALY，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（泳道2）或THOC4/ALY (D3R4R) Rabbit mAb兔单抗 (泳道3)。泳道1为10% input对照。 Western blot 检测中使用的抗体是 THOC4/ALY (D3R4R) Rabbit mAb兔单抗，二抗是 Mouse Anti-rabbit IgG (构象特异) (L27A9) mAb鼠单抗 #3678 。 |
