| 货号 | 5449S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Thymidylate Synthase (D26G11) Rabbit mAb detects endogenous levels of total Thymidylate Synthase protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) is an essential step in the formation of thymine nucleotides (1,2, reviewed in 3). This process is catalyzed by thymidylate synthase (TS or TYMS), a homodimer composed of two 30 kDa subunits. TS is an intracellular enzyme that provides the sole de novo source of thymidylate, making it a required enzyme in DNA biosynthesis with activity highest in proliferating cells (1). Being the exclusive source of dTMP, TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU) (1-5). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head, and neck. Studies have shown that patients with metastases expressing lower levels of TS have a higher response rate to treatment with 5-FU than patients with tumors that have increased levels of TS (5). TS expression may be useful in predicting overall patient survival, but the prognostic value of TS expression continues to be a subject of investigation for different types of cancers (6-10). |
| 存放说明 | -20C |
| 计算分子量 | 30 |
Western blot analysis of extracts from various cell lines using Thymidylate Synthase (D26G11) Rabbit mAb. Wetern blot检测多种细胞系提取物，所用抗体为Thymidylate Synthase (D26G11) Rabbit mAb兔单抗。 | |
Immunoprecipitation of Thymidylate Synthase from Jurkat cell lysate using Thymidylate Synthase (D26G11) Rabbit mAb followed by western blot using the same antibody. Lane 1 is 5% input. 免疫沉淀分析Jurkat细胞裂解液胸苷酸合成酶，所用抗体为Thymidylate Synthase (D26G11) Rabbit mAb兔单抗。1道是5% input. |
